D（6か月以上） B6D2-Dnajb13<em1Osb> B6D2-Dnajb13<em1Osb> D (more than 6 months) human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Dnajb13 gene Dnajb13 mutant mice generated by the CRISPR/Cas9 technique. 2 bp deletion.Dnajb13<em1Osb>, [Deleted sequence]:TAGAGATAGTCTGTTGTGAGATGTCAGGAGCAGAAGGACCTTAGAGGTCGCAAAGCCCTGCCTCCTGTTGCCAAGATGGAAGCAAGAAACTCTGAAGCCTCAGGGAACAGTCTGTGCCTAGGAAGGCAAAATGGGGCCTTGGGTGCCTAGCTTTTGGGGTCCTGCAGGAAGCATCCCTCCAACTCACTTCCCATCATAGCTATAGGATAGGAATCTTTATTATGTCAGCCTAAGGTCTCACGCCTCCCCATCTTCATAGGCTGTGGGAGTTGAGTGGATAGGAGCAGGCAAGGCCCAGCTAGCCCTCTCCATCCCTGTTCCTGCTCAGGTCCCCCAGAATTAGGTCAGCAATGCCTGGCGCAGCATCTGCTTCTTCTGGGGTGTGAGGCGGGTGGGGAACTGGATGTCAAAGAAGATGAAGAGGTCTCCCTTC[TT]GCTGGGGTTCTCAGGCAGTGGCATCCCCTCACCTGGCACAATCTTGAAGTACTTAGGGctgagacagggaaagaagaaaggaaggagcatagatgactcagcagtgtcggatgggtgcacgctacttcctgcacacttgcaggagcctcagccattcttacagcttcccactagctggccaggtctcacacaccagagc 開発者／機関：野田大地，伊川正人／大阪大学開発年：2015雄のDnajb13-2/wt（ヘテロ）と雌のDnajb13 -2/wt（ヘテロ）による兄妹交配により維持している。雄のKOマウスは水頭症のため出生2週間後に死亡。KO精子はimmotile。 RBRC09858 開発者／機関：野田大地，伊川正人／大阪大学 開発年：2015 雄のDnajb13-2/wt（ヘテロ）と雌のDnajb13 -2/wt（ヘテロ）による兄妹交配により維持している。雄のKOマウスは水頭症のため出生2週間後に死亡。KO精子はimmotile。 CRISPR/Cas9システムによりDnajb13遺伝子をヘテロ欠失したマウス (2塩基欠損) 。 true Dnajb13<-2/wt> Dnajb13<-2/wt> Developed by Taichi Noda and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. BDF1 background. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666.<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A>